Development, Linkage Mapping, and Use of Microsatellites in Bermudagrass

作者：Harris Shultz Karen R^*; Schwartz Brian M; Hanna Wayne W; Brady Jeff A

来源：Journal of the American Society for Horticultural Science, 2010, 135(6): 511-520.

DOI：10.21273/jashs.135.6.511

• Cynodon
• EST-SSR
• genetic mapping
• SDAF

Genetic linkage maps of bermudagrass (Cynodon spp) species using 118 triploid individuals derived from a cross of T89 [C dactylon (2n = 4x = 36)] and T574 [C transvaalensis (2n = 2x = 18)1 were enriched with expressed sequence tags-derived simple sequence repeat (EST-SSR) markers Primers were developed from 53 ESTs containing SSRs producing 75 segregating markers from which 28 could be mapped to the T89 and T574 genetic maps With the addition of previously generated marker data, 26 T89 linkage groups and eight T574 linkage groups were formed using a log-of-odds (LOD) value of 4 0 The T89 and T574 linkage maps spanned 1055 cM and 311 1 cM and include 125 and 36 single-dose amplified fragments (SDAFs), respectively Many of the SDAFs displayed disomic segregation and thus T89 may be a segmental allotetraploid or an allotetraploid The additional EST-SSR markers add value to the maps by increasing marker density and provide markers that can be easily transferred to other bermudagrass populations Furthermore, EST-SSRs can be immediately used to assess genetic diversity, identify non-mutated cultivars of bermudagrass, confirm pedigrees, and differentiate contaminants from cultivars derived from 'Tifgreen'

• 出版日期2010-11