A Biphasic Response Pattern of Lipid Metabolomics in the Stage Progression of Hepatitis B Virus X Tumorigenesis

作者：Teng Chiao Fang; Hsieh Wen Chuan; Yang Ching Wen; Su Hui Min; Tsai Ting Fen; Sung Wang Chou; Huang Wenya; Su Ih Jen^*

来源：Molecular Carcinogenesis, 2016, 55(1): 105-114.

DOI：10.1002/mc.22266

• metabolic syndrome
• hepatocellular carcinoma
• triglycerides
• cholesterol
• fatty acids

Metabolic syndrome has closely linked to the development of human hepatocellular carcinoma (HCC). By using the hepatitis B virus (HBV) X (HBx) transgenic mouse model, we studied the dynamic evolution of serum and liver profiles of lipids and global cDNA expression at different stages of HBx tumorigenesis. We observed that the lipid (triglycerides, cholesterol, and fatty acids) profiles revealed a biphasic response pattern during the progression of HBx tumorigenesis: a small peak at early phase and a large peak or terminal switch at the tumor phase. By analyzing cDNA microarray data, the early peak correlated to the oxidative stress and pro-inflammatory response, which then resolved at the middle phase and were followed by the terminal metabolic switch in the tumor tissues. Five lipid metabolism-related genes, the arachidonate 5-lipoxygenase, lipoprotein lipase, fatty acid binding protein 4, 1-acylglycerol-3-phosphate O-acyltransferase 9, and apolipoprotein A-IV were identified to be significantly activated in HBx transgenic HCCs and further validated in human HBV-related HCCs. Inhibition of these lipid genes could reverse the effect of HBx on lipid biosynthesis and suppress HBx-induced cell proliferation in vitro. Our results support the concept that metabolic syndrome plays an important role in HBV tumorigenesis. The dysregulation of lipid metabolic genes may predict the disease progression to HCC in chronic hepatitis B patients.

• 出版日期2016-1