Brainstem facilitations and descending serotonergic controls contribute to visceral nociception but not pregabalin analgesia in rats

作者：Sikandar Shafaq^*; Bannister Kirsty; Dickenson Anthony H

来源：Neuroscience Letters, 2012, 519(1): 31-36.

DOI：10.1016/j.neulet.2012.05.009

• Visceral nociception
• Rostral ventromedial medulla (RVM)
• Visceromotor response (VMR)
• 5-HT
• Pregabalin

Pro-nociceptive ON-cells in the rostral ventromedial medulla (RVM) facilitate nociceptive processing and contribute to descending serotonergic controls. We use RVM injections of neurotoxic dermorphin-saporin (Derm-SAP) in rats to evaluate the role of putative ON-cells, or mu-opioid receptor-expressing (MOR) neurones, in visceral pain processing. Our immunohistochemistry shows that intra-RVM Derm-SAP locally ablates a substantial proportion of MOR and serotonergic cells. Given the co-localization of these neuronal markers, some RVM ON-cells are serotonergic. We measure visceromotor responses in the colorectal distension (CRD) model in control and Derm-SAP rats, and using the 5-HT3 receptor antagonist ondansetron, we demonstrate pro-nociceptive serotonergic modulation of visceral nociception and a facilitatory drive from RVM MOR cells. The alpha(2)delta calcium channel ligand pregabalin produces state-dependent analgesia in neuropathy and osteoarthritis models relating to injury-specific interactions with serotonergic facilitations from RVM MOR cells. Although RVM MOR cells mediate noxious mechanical visceral input, we show that their presence is not a permissive factor for pregabalin analgesia in acute visceral pain.

• 出版日期2012-6-21