Mechanical property analysis of stored red blood cell using optical tweezers

作者：Li Yanjie; Wen Cheng; Xie Huimin^*; Ye Anpei; Yin Yajun

来源：Colloids and Surfaces B: Biointerfaces , 2009, 70(2): 169-173.

DOI：10.1016/j.colsurfb.2008.11.012

• Red blood cell
• Optical tweezers
• Digital image correlation (DIC)
• Deformation
• Force

The deformation of human red blood cells subjected to direct stretching by optical tweezers was analyzed. The maximum force exerted by optical tweezers on the cell via a polystyrene microbead 5 mu m in diameter was 315 pN. Digital image correlation (DIC) method was introduced to calculate the force and the deformation of the cell for the first time. Force-extension relation curves of the biconcave cell were quantitatively assessed when erythrocytes were stored in Alsever's Solution for 2 days, 5 days, 7 days and 14 clays respectively. Experiment results demonstrated that the deformability, of red blood cells was impaired with the stored time.

• 出版日期2009-5-1
• 单位清华大学; 北京大学