科研之友
微信
新浪微博
Facebook
分享链接
摘要
Objective To study the influence of anti-macrophage migration inhibitory factor antibody (anti-MIF) on tumor growth and hepatic metastasis of colorectal cancer.Methods After subcutaneous injection of CT26 cells on the nucha of BALB/C mice,the tumor was formed 10 days later.Orthotopic transplantation with fresh tumor tissues was performed on herniated cecum in 20 BALB/C mice,and the mice were randomly divided into group A and group B.The mice in group A and group B received intraperitoneal injection of anti-MIF and normal saline every other day,respectively.Blood samples were collected by heart puncture and the mice were sacrificed 4 weeks later.The tumor in situ and the liver were observed.The whole liver was made into serial slices for observation of the liver metastasis focus.Enzyme linked immunosorbent assay (ELISA) was used to detect the expression of MIF and MMP-9 in serum of mice.Results The weight of the tumor in situ of group A was much lighter than that of group B [(1.60±0.18) g vs.(2.11±0.25) g,P<0.01].There was no significant difference in liver weight between group A and group B [(0.93±0.07) g vs.(0.96±0.07) g,P>0.05].The incidence of hepatic metastasis in group A was significantly lower than in group B [1/10 vs.7/10,P<0.05].Moreover,the expression of MIF and MMP-9 in serum of group A was markedly lower than in group B,too [(21.15±1.59) ng/L vs.(35.65±1.34) ng/L,P<0.01;(0.19±0.01) μg/L vs.(0.28±0.04) μg/L,P<0.01].Conclusion Anti-MIF can not only obviously inhibit the growth of colorectal cancer but also decrease the incidence of hepatic metastasis.
