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摘要
A multiplex polymerase chain reaction (PCR) combined with denaturing high-performance liquid chromatography (DHPLC) method to simultaneously detect foodborne enterotoxigenic Escherichia coli (ETEC), enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC) and enteroinvasive E. coli (EIEC) were described. Following the design of PCR primer pairs specific for ETEC, EPEC, EHEC and EIEC, respectively, the multiplex PCR was developed, and then PCR products were subjected to DHPLC analysis under nondenaturing condition. The specificity and potential diagnostic capability of the multiplex PCR-DHPLC method were evaluated, and results demonstrated that the multiplex PCR-DHPLC is an effective method to rapidly identify the major four categories of diarrheagenic E. coli, simultaneously.
