Adipose tissue-derived mesenchymal stem cells cultured at high density express IFN-beta and suppress the growth of MCF-7 human breast cancer cells

作者：Ryu Hoon; Oh Ji Eun; Rhee Ki Jong; Baik Soon Koo; Kim Jiye; Kang Seong Joon; Sohn Joon Hyung; Choi Eunhee; Shin Ha Cheol; Kim Yong Man; Kim Hyun Soo; Bae Keum Seok^*; Eom Young Woo

来源：Cancer Letters, 2014, 352(2): 220-227.

DOI：10.1016/j.canlet.2014.06.018

• Adipose tissue-derived stem cells
• MCF-7
• IFN-beta
• High density culture
• Growth suppression

Although it has been reported that mesenchymal stem cells (MSCs) suppress tumor growth in vitro and in vivo, little is known about the underlying molecular mechanisms. We found that type I interferon is expressed in adipose tissue-derived stem cells (ASCs) cultured at high density, and ASCs and their conditioned medium (ASC-CM) suppress the growth of MCF-7 cells in vitro. Growth inhibition was amplified by glucose deprivation that resulted from high density culture of ASCs after 3 days. The cytotoxic effect of the ASC-CM obtained from high density culture of ASCs was neutralized by anti-IFN-beta antibody. STAT1 was phosphorylated in MCF-7 cells treated with ASC-CM, and JAK1/JAK2 inhibitor treatment decreased STAT1 phosphorylation. The cytotoxic effect of ASC-CM was reduced especially by JAK1 inhibitors in MCF-7 cells. Our findings suggest that ASCs cultured at high density express type I interferons, which suppresses tumor growth via STAT1 activation resulting from IFN-beta secretion in MCF-7 breast cancer cells.

• 出版日期2014-10-1