Analysis of silica nanoparticles by capillary electrophoresis coupled to an evaporative light scattering detector

作者：Adelantado Carlos; Rodriguez Farinas Nuria; Rodriguez Martin Doimeadios Rosa C; Zougagh Mohammed; Rios Angel^*

来源：Analytica Chimica Acta, 2016, 923: 82-88.

DOI：10.1016/j.aca.2016.03.055

• Capillary electrophoresis
• Evaporative light scattering detector
• Coupling arrangement
• Size separation
• Silica nanoparticles
• Salt samples

A simple and rapid methodology has been developed to identify and separate silica nanoparticles (SiO(2)NPs) of different sizes in aqueous solution by capillary zone electrophoresis coupled to an evaporative light scattering detector (CE-ELSD). SiO(2)NPs were separated using 3 mM ammonium acetate buffer, containing 1% methanol at pH 6.9. SiO(2)NPs of 20, 50 and 100 nm were successfully separated under the optimum experimental conditions. CE coupled to ELSD has been proven to be an effective separation technique to determine particles with such small sizes, although the peaks are very close to each other, and it is a promising technique that may allow the separation of other types of nanoparticles. Confirmation by TEM and quantification of the SiO2 content was also carried out by inductively coupled plasma-mass spectrometry (ICP-MS). The new method was applied to the analysis of real samples, in order to assess its ability to avoid matrix effects in the determination of SiO(2)NPs in these kinds of samples.

• 出版日期2016-6-7
• 单位中国科学院长春应用化学研究所