A method for determining alkaline phosphatase activity in marine phytoplankton using spectrofluorometry

作者：Peacock Melissa Blakely^*; Kudela Raphael M

来源：Journal of Microbiological Methods, 2012, 89(3): 209-212.

DOI：10.1016/j.mimet.2012.03.007

• Alkaline phosphatase
• Enzyme-labeled fluorescence
• Phytoplankton
• Spectrofluorometry
• Flow cytometry
• Epifluorescent microscopy

A method for determining relative percent intensity alkaline phosphatase activity (APA) using enzyme labeled fluorescence coupled with spectrofluorometry is presented. Compared to traditional microscopy and flow cytometry, we increase statistical power and reduce sample-handling issues. Combined with a biological standard, our method can quantify APA of natural plankton assemblages.

• 出版日期2012-6