科研之友
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摘要
A patch-type oxygen imaging sheet useful for in vitro cellular metabolic assays was developed. Oxygen-responsive fluorescent microbeads were embedded into a biocompatible polyacrylamide gel sheet, which can be directly attached onto target cells for fluorescent imaging of metabolic activity. The sensor beads were directed in a microfluidic device using AC and DC electric manipulation techniques, followed by encapsulation in a hydrogel. Fluorescent imaging of oxygen-consuming activity was demonstrated for glucose oxidase-modified microparticles as cellular models to show the applicability of the imaging sheet to bioassays.
- 出版日期2012-5
