Autologous HIV-1 Clade-B Nef Peptides Elicit Increased Frequency, Breadth and Function of CD8(+) T-Cells Compared to Consensus Peptides

作者：Doroudchi Mehrnoosh; Yegorov Oleg; Baumgartner Tom; Kernaleguen Anne Elen; Breton Gaelle; Ndongala Michel L; Boulassel Mohamed Rachid; Routy Jean Pierre; Bernard Nicole F; Sekaly Rafick Pierre; Yassine Diab Bader^*

来源：PLos One, 2012, 7(11): e49562.

DOI：10.1371/journal.pone.0049562

Objective: To determine the function and phenotype of CD8(+) T-cells targeting consensus and autologous sequences of entire HIV-1 Nef protein. %26lt;br%26gt;Methods: Multiparameter flow cytometry-based analysis was used to evaluate the responses of two treatment naive HIV-infected individuals, during primary and the chronic phases of infection. %26lt;br%26gt;Results: A greater breadth and magnitude of CD8 IFN-gamma responses to autologous compared to clade-B consensus peptides was observed in both subjects. Cross recognition between autologous and consensus peptides decreased in both subjects during progression from primary to chronic infection. The frequencies of TEMRA and TEM CD8(+) T-cells targeting autologous peptides were higher than those targeting consensus peptides and were more polyfunctional (IFN-gamma(+) Gr-B+ CD107a(+)). %26lt;br%26gt;Conclusions: Our data indicate superior sensitivity and specificity of autologous peptides. The functional and maturational aspects of %26quot;real%26apos;%26apos; versus %26quot;cross-recognized%26apos;%26apos; responses were also found to differ, highlighting the importance of a sequence-specific approach towards understanding HIV immune response.

• 出版日期2012-11-19