A regulatory circuit of two lncRNAs and a master regulator directs cell fate in yeast

作者：Moretto Fabien; Wood N Ezgi; Kelly Gavin; Doncic Andreas; van Werven Folkert J

来源：Nature Communications, 2018, 9(1): 780.

DOI：10.1038/s41467-018-03213-z

<jats:title>Abstract</jats:title><jats:p>Transcription of long noncoding RNAs (lncRNAs) regulates local gene expression in eukaryotes. Many examples of how a single lncRNA controls the expression of an adjacent or nearby protein-coding gene have been described. Here we examine the regulation of a locus consisting of two contiguous lncRNAs and the master regulator for entry into yeast meiosis, <jats:italic>IME1</jats:italic>. We find that the cluster of two lncRNAs together with several transcription factors form a regulatory circuit by which <jats:italic>IME1</jats:italic> controls its own promoter and thereby promotes its own expression. Inhibition or stimulation of this unusual feedback circuit affects timing and rate of <jats:italic>IME1</jats:italic> accumulation, and hence the ability for cells to enter meiosis. Our data demonstrate that orchestrated transcription through two contiguous lncRNAs promotes local gene expression and determines a critical cell fate decision.</jats:p>

• 出版日期2018-2-22