microRNA-497-5p targeted SOX9 to inhibit proliferation, migration and invasion of glioma cells

作者：Yan, Yu; Peng, Yong; Ou, Yangzhu; Jiang, Yugang^*

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(8): 8027-8036.

• miR-497-5p
• SOX9
• proliferation
• migration
• invasion
• glioma

Increasing studies indicate that deregulation of microRNAs (miRNAs) contributes to the carcinogenesis and progression of glioma. The present study was aimed to investigate the roles of miR-497-5p in the progression of glioma and to elucidate underlying miR-497-5p-mediated molecular mechanisms in glioma. Our results showed that miR-497-5p was significantly down-regulated in glioma tissues and cell lines. Enforced miR-497-5p expression inhibited cell proliferation, migration and invasion of glioma cells. Moreover, bioinformatics analysis predicated that SRY-related high mobility group-Box gene 9 (SOX9) was a putative target of miR-497-5p. By using luciferase reporter assay, qRT-PCR and western blot, we demonstrated that SOX9 was a direct target gene of miR-497-5p in glioma. We also found that knockdown of SOX9 could simulate the suppressive functions of miR-497-5p in glioma. Taken together, these findings suggested that miR-497-5p functioned as a tumor suppressor in glioma by targeting SOX9. It might be a novel therapeutic target for patients with glioma.

• 出版日期2016
• 单位中南大学