Gene Segment Reassortment Between American and Asian Lineages of Avian Influenza Virus from Waterfowl in the Beringia Area

作者：Wahlgren J; Waldenstrom J; Sahlin S; Haemig P D; Fouchier R A M; Osterhaus A D M E; Pinhassi J; Bonnedahl J; Pisareva M; Grudinin M; Kiselev O; Hernandez J; Falk K I; Lundkvist A; Olsen B^*

来源：Vector Borne and Zoonotic Diseases, 2008, 8(6): 783-790.

DOI：10.1089/vbz.2007.0274

• Avian influenza
• Eurasian lineage
• American lineage

Since prehistoric times, the Bering Strait area (Beringia) has served as all avenue of dispersal between the Old and the New Worlds. On a field expedition to this area, we collected fecal samples from dabbling ducks, geese, shorebirds, and gulls on the Chukchi Peninsula, Siberia, and Pt. Barrow, Alaska, and characterized the sub-types of avian influenza virus present in them. Four of 202 samples (2%) from Alaska were positive for influenza A virus RNA in two independent polymerase chain reaction (PCR)-based screening assays, while all shorebird samples from the Chukchi Peninsula were negative. Subtypes H3N8 and H6N1 were recorded once, while subtype H8N4 was found in two samples. Full-length sequences were obtained from the three unique isolates, and phylogenetic analysis with representative sequences for the Eurasian and North American lineages of influenza A virus showed that one HA gene Clustered with the Eurasian rather than the North American lineage. However, the closest relative to this sequence was a North American isolate from Delaware described in 2002, indicating that a H6 spillover from Asia has established itself in North America.

• 出版日期2008-12