Molecular identification of weedy Sporobolus species by PCR-RFLP

作者：Shrestha S^*; Graham G C; Loch D S; Adkins S W

来源：Weed Research, 2010, 50(5): 383-387.

DOI：10.1111/j.1365-3180.2010.00806.x

• diagnostics
• giant rats tail grasses
• internal transcribed spacers (ITS)
• polymerase chain reaction
• ribosomal DNA
• ITS sequences

P>Identification of the five noxious weedy Sporobolus species from non-weedy and native species in Australia is extremely difficult. Furthermore, seeds of these species, which are found in commercial seed lots, are morphologically indistinguishable. The lack of a reliable identification protocol is contributing to the rapid spread of these noxious weedy species within Australia. A polymerase chain reaction-restriction fragment length polymorphism approach carried out on nuclear ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequences was used to create a molecular diagnostic tool. To develop this tool, 40 ITS sequences generated from 14 Sporobolus species were used. A restriction enzyme (MvnI) was identified which could cut the ITS sequences from the noxious weedy species into two approximately equal fragments, while not cutting those from all other species. When tested on 23 randomly selected seed samples, identification was accomplished with 100% accuracy, suggesting a possible application in seed certification programmes.

• 出版日期2010-10