Antibacterial and Anti-Inflammatory Activities of Physalis Alkekengi var. franchetii and Its Main Constituents

作者：Shu, Zunpeng; Xing, Na; Wang, Qiuhong^*; Li, Xinli; Xu, Bingqing; Li, Zhenyu; Kuang, Haixue^*

来源：Evidence-Based Complementary and Alternative Medicine, 2016, 2016: 4359394.

DOI：10.1155/2016/4359394

• NF-KAPPA-B
• ANTIMICROBIAL ACTIVITY
• GROUPS C
• INFLAMMATION
• PHARYNGITIS

This study was designed to determine whether the 50% EtOH fraction from AB-8 macroporous resin fractionation of a 70% EtOH extract of P. Alkekengi (50-EFP) has antibacterial and/or anti-inflammatory activity both in vivo and in vitro and to investigate the mechanism of 50-EFP anti-inflammatory activity. Additionally, this study sought to define the chemical composition of 50-EFP. Results indicated that 50-EFP showed significant antibacterial activity in vitro and efficacy in vivo. Moreover, 50-EFP significantly reduced nitric oxide (NO), prostaglandin E-2 (PGE(2)), tumor necrosis factor alpha (TNF-alpha), interleukin 1 (IL-1), and interleukin 6 (IL-6) production in lipopolysaccharide-(LPS-) stimulated THP-1 cells. Nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) (examined at the protein level) in THP-1 cells were suppressed by 50-EFP, which inhibited nuclear translocation of p65. Consistent with this anti-inflammatory activity in vitro, 50-EFP reduced inflammation in both animal models. Finally, seventeen compounds (8 physalins and 9 flavones) were isolated as major components of 50-EFP. Our data demonstrate that 50-EFP has antibacterial and anti-inflammatory activities both in vitro and in vivo. The anti-inflammatory effect appears to occur, at least in part, through the inhibition of nuclear translocation of p65. Moreover, physalins and flavones are probably the active components in 50-EFP that exert antibacterial and anti-inflammatory activities.

• 出版日期2016
• 单位大连医科大学; 黑龙江中医药大学