Dynamic Changes of the Frequency of Classic and Inflammatory Monocytes Subsets and Natural Killer Cells in Chronic Hepatitis C Patients Treated by Direct-Acting Antiviral Agents

作者：Ning, Gang; Li, Yi-ting; Chen, You-ming; Zhang, Ying; Zeng, Ying-fu; Lin, Chao-shuang^*

来源：Canadian Journal of Gastroenterology and Hepatology, 2017, 2017: 3612403.

DOI：10.1155/2017/3612403

• SUSTAINED VIROLOGICAL RESPONSE
• INTERFERON-FREE THERAPY
• VIRUS-INFECTION
• HEPATOCELLULAR-CARCINOMA
• GENOTYPE 1
• OPEN-LABEL
• RECURRENCE
• SOFOSBUVIR
• RIBAVIRIN
• SAFETY

Objective. Up to now, little was known about the immunological changes of chronic hepatitis C (CHC) patients treated with directacting antiviral agents (DAAs); we try to explore the effect of DAAs on the frequency of monocytes, NK cells, and cytokines that promote their activation. Methods. 15 treatment-naive CHC patients and 10 healthy controls were recruited. Patients were examined before DAAs therapy (0w) and at week 4 (4w) and week 12 (12w) of therapy. Percentage of monocytes and NK cells of the peripheral blood was analyzed by flow cytometry. Serum cytokines IL-12, IL-18, CXCL10, CXCL11, sCD14, and sCD163 were measured by enzyme linked immunosorbent assay. Results. The frequency of CD3-CD16(+) CD56(+) NKcells and classicCD14(++) CD16 monocytes decreased, while CD14(+) CD16(+) monocytes and cytokines IL-12, IL-18, CXCL10, CXCL11, sCD14, and sCD163 increased at 0 w compared to healthy controls. During DAAs treatment, the decreased NK cells and classic monocytes gradually increased to normal levels; the increased inflammatory monocytes and cytokines IL-12 and CXCL11 decreased to normal levels, but the increased cytokines IL-18, CXCL10, sCD14, and sCD163 still remained at high levels at 12 w though they decreased rapidly from 0w. Conclusion. Our results showed that DAAs treatment attenuated the activation of monocytes and NK cells in CHC patients.

• 出版日期2017
• 单位中山大学