Altered chemokine receptor expression in the peripheral blood lymphocytes in polymyositis and dermatomyositis

作者：Zhu, Ziwei; Yang, Chunshu; Wang, Jianing; Feng, Qianhui; Chen, Qi; Yang, Pingting^*

来源：Cytokine, 2017, 99: 316-321.

DOI：10.1016/j.cyto.2017.08.018

• Chemoattractant
• CD4(+) T cell
• CD8(+) Tcell
• Th1/Th2
• Chemokine receptor

Objective: To examine the expression of chemokine receptors in different peripheral blood T-cell subsets in patients with polymyositis (PM) and dermatomyositis (DM).
Methods: We used flow cytometry to measure the frequencies of chemokine receptors CXCR3 and CCR4 expression in the CD4(+) or CD8(+) lymphocytes. Enzyme linked immunosorbent assays were also used to measure the concentrations of C-X-C modf chemokine 10 (CXCL10), thymus and activation regulated chemokine (TARC) and macrophage derived chemokine (MDC).
Results: Comparing to 20 healthy controls, %CD4(+)CXCR3(+) and %CD8(+)CXCR3(+) T cells significantly decreased in 33 DM patients, and %CD8(+)CXCR3(+)cells decreased in 24 PM patients, but %CD4(+)CCR4(+) and %CD8(+)CCR4(+) cells did not significantly change in both the PM and DM patients. Accordingly, the Th1/Th2 polarization, analyzed as the balance obtained after dividing %CD4(+)CXCR3(+) cells by %CD4(+)CCR4(+) cells, showed a significant reduction in DM. The serum concentration of CXCR3(+) ligand, CXCL10, significantly increased and negatively correlated with circulating %CD4(+)CXCR3(+) cells in DM patients. There was no significant change of TARC and MDC in PM and DM patients. Furthermore, %CD4(+)CXCR3(+) cells decreased more severely in the patients with interstitial lung disease.
Conclusions: The present results indicate that the distributions of circulating CXCR3(+) T-cells differ among the PM and DM cases. Our findings suggest a pathogenic difference between PM and DM.

• 出版日期2017-11
• 单位中国医科大学