Molecular tagging and mapping of a major linked marker for taproot thickness in a Brassica crop

作者：Cao, J. S.; Lu, G.^*; Jiang, L. J.; Chen, L.; Xiang, X.; Chen, H.

来源：Journal of Horticultural Science & Biotechnology, 2009, 84(2): 175-180.

DOI：10.1080/14620316.2009.11512500

• BULKED SEGREGANT ANALYSIS
• QUANTITATIVE TRAIT LOCI
• CONTROLLING ROOT TRAITS
• ORYZA-SATIVA L.
• RAPA L.
• ASSISTED SELECTION
• SYN. CAMPESTRIS
• DAUCUS-CAROTA
• QTLS
• RICE

A DNA marker linked to the locus controlling storage-root thickness was identified using bulked segregant analysis (BSA) in an F-2 population derived from a cross between turnip (Brassica rapa L. ssp. rapifera Matzg.) and rapeseed (Brassica rapa L.). Root-thickness in the F-2 plants showed a continuous distribution from 2.2 - 10.7 cm, suggesting the effects of a few major and minor genes. BSA, using three bulks based on root-thickness, suggested that an AFLP marker (EACCMCAG-220) which appeared only in plants with a thickened root, was linked to a locus that controlled root size. This AFLP band was cloned, sequenced, and successfully converted into a simple, dominant, sequence characterised amplified region (SCAR) marker. This marker may help to identify turnip genotypes with thicker taproots in segregating progenies of root crops during marker-assisted selection (MAS) breeding programmes.

• 出版日期2009-3
• 单位北京市农林科学院; 浙江大学