Antioxidant potential assessment of phenolic and flavonoid rich fractions of Clematis orientalis and Clematis ispahanica (Ranunculaceae)

作者：Karimi Ehsan; Nohooji Majid Ghorbani^*; Habibi Meisam; Ebrahimi Mandi; Mehrafarin Ali; Khalighi Sigaroodi Farahnaz

来源：Natural Product Research, 2018, 32(16): 1991-1995.

DOI：10.1080/14786419.2017.1359171

• Clematis orientalis
• Clematis ispahanica
• antioxidant activity
• HPLC

The antioxidant activities of crude extract fractions using Hexane, Chloroform, Ethyl Acetate, Butanol and Water of Clematis orientalis and Clematis ispahanica were investigated. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and the ferric reducing/antioxidant potential (FRAP) were used to evaluate the antioxidant capacity. The total phenolics were found to be 4.37-9.38 and 1.32-11.37 mg gallic acid equivalents (GAE)/g in different fractions for C. orientalis and C. ispahanica, respectively. The ethyl acetate fraction of C. orientalis and chloroform fraction of C. ispahanica showed the highest DPPH and FRAP activities at a concentration of 300 mu g/mL.The predominant phenolic compounds identified by HPLC in C. orientalis were Resorcinol (603.5 mu g/g DW) in chloroform fraction and Ellagic acid (811.7 mu g/g DW) in chloroform fraction of C. ispahanica.
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• 出版日期2018
• 单位河北医科大学