科研之友
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摘要
The gene encoding the dihydropteroate synthase (DHPS) of Streptococcus pneumonia has been cloned, sequenced, and expressed in Escherichia coli. The protein has been purified and used to develop a novel microplate assay for the detection of sulfonamides. The assay was based on the competition between sulfonamides and horseradish peroxidase (HRP)-labeled sulfonamide derivative, 4-(4-aminobenzenesulfonylamino) benzoic acid (CS) for the immobilized protein. Under optimized conditions, nine sulfonamides and p-aminobenzoic acid (PABA) could be detected below 100 ng/ml and 28 sulfonamides used in the study could be detected with IC50 values ranging from 426 to 50,000 ng/ml. It is concluded that this method offers a robust and rapid alternative to other methods for the screening of sulfonamides.
- 出版日期2013-7-15
- 单位中国农业大学
