Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

作者：Pongsomboon Siri****; Tang Sureerat; Boonda Sulee****; Aoki Takashi; Hirono Ikuo; Yasuike Motoshige; Tassanakajon Anchalee^*

来源：BMB Reports, 2008, 41(9): 670-677.

• DNA microarray
• gene expression
• Penaeus monodon
• real-time RT-PCR
• yellow head virus

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly (<= 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by real-time RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.

• 出版日期2008-9-30