Mechanisms underlying protective effects of trimetazidine on endothelial progenitor cells biological functions against H2O2-induced injury: Involvement of antioxidation and Akt/eNOS signaling pathways

作者：Wu, Qinqin; Qi, Benling^*; Liu, Yun; Cheng, Bei; Liu, Lihua; Li, Yuanyuan; Wang, Qian

来源：European Journal of Pharmacology, 2013, 707(1-3): 87-94.

DOI：10.1016/j.ejphar.2013.03.027

• Trimetazidine
• Endothelial progenitor cells
• Oxidative stress
• Akt/eNOS signaling pathway
• Ischemic heart disease

Trimetazidine (TMZ) is a widely used drug exerting cardioprotective effects against ischemic heart disease through a number of mechanisms in conditions of oxidative stress. However, there are few data regarding the effects of TMZ on endothelial lineage, especially endothelial progenitor cells (EPCs). Thus, we sought to investigate whether TMZ could protect EPCs against oxidative stress injury induced by H2O2 (100 mu M) and the preliminary mechanisms involved in vitro. The results showed that pretreatment of EPCs with TMZ (10 mu M) protected the proliferation, adhesion, migration, and apoptosis of EPCs against H2O2, accompanied by an increase in superoxide dismutase (SOD) activity, a decrease in malonaldehyde (MDA) content, and increases in eNOS, Akt phosphorylation, and NO production. These TMZ-mediated beneficial effects on EPCs could be attenuated by pre-incubation with the Akt inhibitor triciribine. In conclusion, the present study demonstrates that TMZ ameliorated H2O2-induced impairment of biological functions in EPCs with the involvement of antioxidation and Akt/eNOS signaling pathway. These findings suggest that TMZ mediating preservation of EPCs may contribute to its cardioprotective effects on ischemic heart disease.

• 出版日期2013-5-5
• 单位华中科技大学