Hepatoprotective Properties of a Polyphenol-Enriched Fraction from Annona crassiflora Mart. Fruit Peel against Diabetes-Induced Oxidative and Nitrosative Stress

作者：Justino Allisson Benatti; Pereira Mariana Nunes; Peixoto Leonardo Gomes; Vilela Danielle Diniz; Caixeta Douglas Carvalho; de Souza Adriele Vieira; Teixeira Renata Roland; Guerra Silva Heitor Cappato; Rosa de Moura Francyelle Borges; Moraes Izabela Barbosa; Espindola Foued Salmen^*

来源：Journal of Agricultural and Food Chemistry, 2017, 65(22): 4428-4438.

DOI：10.1021/acs.jafc.7b01355

• Annonaceae
• antioxidant
• liver
• natural products
• nitrosative stress
• oxidative stress

A polyphenol-enriched fraction from Annona crassiflora fruit peel (Ac-Pef) containing chlorogenic acid, (epi)catechin, procyanidin B2, and caffeoyl-glucoside was investigated against hepatic oxidative and nitrosative stress in streptozotocin-induced diabetic rats. Serum biochemical parameters, hepatic oxidative and nitrosative status, glutathione defense system analysis, and in silico assessment of absorption, distribution, metabolism, excretion, and toxicity (ADMET) of the main compounds of Ac-Pef were carried out. Ac-Pef treatment during 30 days decreased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities, as well as hepatic lipid peroxidation, protein carbonylation and nitration, inducible nitric oxide synthase level, and activities and expressions of glutathione peroxidase, superoxide dismutase, and catalase. There were increases in antioxidant capacity, glutathione reductase activity, and reduced glutathione level. ADMET predictions of Ac-Pef compounds showed favorable absorption and distribution; with no hepatotoxicity. A. crassiflora fruit peel showed hepatoprotective properties, indicating a proftrising natural source of bioactive molecules for prevention and therapy of diabetes complications.

• 出版日期2017-6-7