A sensitive electrochemical aptasensor for highly specific detection of streptomycin based on the porous carbon nanorods and multifunctional graphene nanocomposites for signal amplification

作者：Yin, Junling; Guo, Wenjuan^*; Qin, Xiaoli; Zhao, Juan; Pei, Meishan; Ding, Feng^*

来源：Sensors and Actuators B: Chemical , 2017, 241: 151-159.

DOI：10.1016/j.snb.2016.10.062

• Electrochemical aptasensor
• Porous carbon nanorods
• Ferriferrous oxide nanoparticles
• Graphene
• Streptomycin

Quantitative detection of antibiotic residues in animal food stuffs is of great significance. In this work, a highly sensitive electrochemical aptasensor for the sensitive detection of streptomycin antibiotic was fabricated based on a novel signal amplification strategy. Specifically, this aptasensor was constructed utilizing porous carbon nanorods (PCNR) formed by porous carbon nanosphere and multifunctional graphene composite (GR-Fe3O4-AuNPs) as biosensing substrate. PCNR samples with large specific pore volume and high specific surface area were successfully prepared by hydrothermal and chemical activation treatment for the first time. GR-Fe3O4-AuNPs was served as labels to achieve a high sensitivity and low limit of detection (LOD). Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to streptomycin in the range 0.05-200 ng/mL with a low detection limit of 0.028 ng/mL. The proposed aptasensor displayed an excellent analytical performance with great reproducibility, high selectivity and stability. In addition, the as-prepared aptasensor was successfully utilized for the determination of streptomycin in real samples.

• 出版日期2017-3-31
• 单位济南大学