A comparative study of three different synthesis routes for hydrophilic fluorophore-doped silica nanoparticles

作者：Shahabi Shakiba; Treccani Laura^*; Rezwan Kurosch

来源：Journal of Nanoparticle Research, 2016, 18(1): 28.

DOI：10.1007/s11051-016-3334-0

• Fluorescence nanoparticle
• Microemulsion
• Seeds regrowth
• Silica
• Stober
• Biomedical imaging

The synthesis of fluorophore-doped silica nanoparticles (FDS NPs) with two conventional approaches, Stober and microemulsion, as well as a novel amino acid-catalyzed seeds regrowth technique (ACSRT) is presented. The efficiency of each applied synthesis route toward incorporation of selected hydrophilic fluorophores, including rhodamine B isothiocyanate and fluorescein isothiocyanate, without and with an amine-containing crosslinker, into silica matrix was systematically studied. Our results clearly highlight the advantages of ACSRT to obtain FDS NPs with a remarkable encapsulation efficiency, high quantum yield, and enhanced stability against bleaching and dye leaking due to efficient embedding of the dyes inside silica network even without the aminecontaining silane reagent. Moreover, evaluation of photostability of FDNPs internalized in human bone cells demonstrates the merits of ACSRT.

• 出版日期2016-1-20