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摘要
Compared to traditional methods, the enzymatic degumming shows the advantages of a clear reaction substrate, mild reaction conditions, low energy consumption and reduced environmental pollution. Free phospholipase A(1) (PLA(1)) was immobilized on magnetic Fe3O4/SiOx-g-P (GMA) nanoparticles to obtain a nanomagnetic immobilized PLA(T) with a particle size of 100.50 +/- 1.3 nm and an activity of 2150 +/- 25 U/g. The Eulerian-Lagrangian model with particles as discrete phase and liquid as continuous phase was selected. The motion law of nanomagnetic enzyme particles in liquid-solid magnetic fluidized bed was simulated. Under the conditions of a magnetic field intensity of 0.022 T and a flow rate of soybean crude oil of 0.0025 m/s, the distribution status of nanomagnetic enzyme in the magnetic fluidized enhanced the enzymolysis reaction. Using the main parameters obtained by numerical simulation, soybean crude oil was degummed in a multistage magnetic fluidized bed. Under the conditions of a magnetic enzyme dosage of 0.10 g/kg, a reaction temperature of 63 degrees C and a reaction system pH of 6.3, after 6.0 h of reaction, the residual phosphorus contents in the oil was 8.2 mg/kg, and the relative activity of the nanomagnetic enzyme was still 86%. The optimized conditions improved significantly the efficiency and stability of the enzymolysis reaction of nanomagnetic enzyme.
