Antioxidative and free radical scavenging effects of ecdysteroids from Serratula strangulata

作者：Cai YJ; Dai JQ; Fang JG; Ma LP; Hou LF; Yang L; Liu ZL^*

来源：Canadian Journal of Physiology and Pharmacology, 2002, 80(12): 1187-1194.

DOI：10.1139/Y02-152

• ecdysteroid
• Serratula strangulata
• free radical
• erythrocyte
• lipid peroxidation
• antioxidant

The antioxidative and free radical scavenging effects of four ecdysteroids, 20-hydroxyecdysone (E1), 25-deoxy-11,20-dihydroxyecdysone (E2), 24-(2-hydroxyethyl)-20-hydroxyecdysone (E3), and 20-hydroxyecdysone-20,22-monoacetonide (E4), isolated from the Chinese herb Serratula strangulata have been investigated in vitro. These ecdysteroids could protect human erythrocytes against oxidative hemolysis induced by a water-soluble azo initiator 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH). They could also inhibit the peroxidation of rat liver microsomes induced by hydroxyl radicals, as monitored by the formation of thiobarbituric acid reactive substances (TBARS), and prevent radical-induced decrease of membrane fluidity as determined by fluorescence polarization. They reacted with galvinoxyl radicals in homogeneous solution, and the pseudo-first-order rate constants were determined spectrophotometrically by following the disappearance of galvinoxyl radicals. Compounds E1 and (or) E3 were the most active in both antioxidative and radical-scavenging reactions.

• 出版日期2002-12
• 单位兰州大学