Autodisplay of an avidin with biotin-binding activity on the surface of Escherichia coli

作者：Pardave Alejandre H D; Alvarado Yaah J E; Pompa Mera E N; Munoz Medina J E; Sarquiz Martinez B; Santacruz Tinoco C E; Manning Cela R G; Ortiz Navarrete V; Lopez Macias C; Gonzalez Bonilla C R^*

来源：Biotechnology Letters, 2018, 40(3): 591-600.

DOI：10.1007/s10529-018-2507-6

• Autodisplay
• Avidin
• Biotin-binding activity
• In trans surface display
• ShdA

To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli.
Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5 alpha. One fusion protein contained 476 amino acids of the ShdA alpha and beta domains, whereas the second consisted of a 314 amino acid from alpha and truncated beta domains. Protein production was verified by SDS-PAGE using an antibody to the molecular FLAG-tag. The surface display of the avidin-shdA fusion protein was confirmed by confocal microscopy and flow cytometry analysis, and the biotin-binding activity was evaluated by fluorescence microscopy and flow cytometry using biotin-4-fluorescein and biotinylated-ovalbumin (OVA).
Expression of a recombinant avidin with biotin-binding activity on the surface of E. coli was achieved using the autotransporter ShdA. This system is an alternative to bind biotinylated molecules to E. coli.

• 出版日期2018-3