科研之友
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摘要
New pathogenic isolates of the Marek's disease virus type 1 (MDV1) were found in various Russian regions. The most variable region of the glycoprotein B (gB) gene was analyzed in 13 MDV strains and isolates and proved to be conserved. Pathogenic strains of MDV1 had two or three 132-nt inverted repeats, in contrast to nonpathogenic strains and MDV type 2 and 3. A highly sensitive complex method was elaborated for detecting MDV and distinguishing between MDV types and the turkey herpesvirus and between pathogenic (oncogenic) and nonpathogenic MDV strains. The method is based on PCR with primers to the gB and thymidine kinase genes and to the MDV inverted repeats.
- 出版日期1998-10
