Detergent quantification in membrane protein samples and its application to crystallization experiments

作者：Prince Chelsy C; Jia Zongchao^*

来源：Amino Acids, 2013, 45(6): 1293-1302.

DOI：10.1007/s00726-013-1600-3

• Crystallography
• Membrane proteins
• Detergent
• Quantification

The structural characterization of membrane proteins remains a challenging field, largely because the use of stabilizing detergents is required. Researchers must first select a suitable detergent for the solubility and stability of their protein during in vitro studies. In addition, an appropriate concentration of detergent in membrane protein samples can be essential for protein solubility, stability, and experimental success. For example, in membrane protein crystallography, detergent concentration in the crystallization drop can be a critical parameter influencing crystal growth. Over the past decade, multiple techniques have been developed for the measurement of detergent concentration using a wide variety of strategies. These methods include colorimetric reactions, which target specific detergent classes, and analytical techniques applicable to a wide variety of detergents. This review will summarize and discuss the available options. It will be a useful resource to those selecting a strategy that best fits their experimental requirements and available instruments.

• 出版日期2013-12