Neutrophil gelatinase associated lipocalin (NGAL) and calprotectin in equine laminar tissue after jejunal obstruction, treated or not with hidrocortisone

作者：Laskoski Luciane M^*; Valadao Carlos A A; Vasconcelos Rosemere O; Faleiros Rafael R; Mendes Heloisa M F; Ferrucci Danilo; Silva Juliete A F; Machado Dagmar D R S

来源：Pesquisa Veterinaria Brasileira, 2012, 32(9): 817-823.

DOI：10.1590/S0100-736X2012000900001

• Horse
• laminitis
• calprotectin
• NGAL
• jejunal obstruction

Laskoski L.M., Valadao C.A.A., Vasconcelos R.O., Faleiros R.R., Mendes H.M.F., Ferrucci D., Silva J.A.F. %26 Machado D.D.R.S. 2012. [Neutrophil gelatinase associated lipocalin (NGAL) and calprotectin in equine laminar tissue after jejunal obstruction, treated or not with hidrocortisone.] Lipocalina associada a gelatinase de neutrofilos (NGAL) e calprotectina no tecido laminar de equinos apos obstrucao jejunal, tratados ou nao com hidrocortisona. Pesquisa Veterinaria Brasileira 32(9):817-823. Departamento de Clinica e Cirurgia Veterinaria, Faculdade de Ciencias Agrarias e Veterinarias, Universidade Estadual Paulista, Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884-900, Brazil. E-mail: [e-mail] %26lt;br%26gt;Laminitis is a severe hoof condition in horses that may cause intense suffering. In this study, leukocyte infiltration in hoof laminar tissue was investigated in horses subject to intestinal obstruction using immunohistochemistry to detect calprotectin, and zymography to detect neutrophil gelatinase associated lipocalin (NGAL). There were four groups: the Control Group (Gc), with seven horses, without surgical procedures; the Sham-operated Group (Gi), with five horses that were subjected to surgical procedure without intestinal obstruction; the No Treat group (Gnt), with four horses subjected to intestinal obstruction (jejunal distention using an intraluminal balloon) without treatment; and Treated group (Gt), with four horses subjected to intestinal obstruction and treated with hydrocortisone. Positive calprotectin imunostaining was detected in all experimental groups, with increase cell counts in horses of the distended group compared with the control group. NGAL expression was increased in Gd compared with Gc e Gi. The Gt did not differ from the others. In conclusion, small intestine distension can promote leukocyte in filtration in equine hoof laminar tissue, and NGAL zymography was considered a useful method for leukocyte tissue detection in horses. New studies will be conducted to verify the possible beneficial anti-inflammatory effects of hydrocortisone in hoof of horses with intestinal obstruction.

• 出版日期2012-9