UPLC fingerprint analysis and identification of flavonoids in Scutellaria indica by UFLC-triple-quadrupole time-of-flight mass spectrometry

作者：Pan, Haifeng^*; Pan, Weidong; Zhao, Shengnan; Du, Yilong; Li, Yanrong; Yang, Mingyu

来源：Journal of Liquid Chromatography & Related Technologies, 2017, 40(7): 361-368.

DOI：10.1080/10826076.2017.1306559

• Flavonoids
• fragmentation regularities
• LC-MS/MS
• Scutellaria indica
• UPLC fingerprint

The aim of this study was to raise the quality control level of Scutellaria indica. A quick and stable ultra performance liquid chromatography method was established for the fingerprint analysis of S. indica. A total of 32 common peaks were marked with 10 batches S. indica detected in 30min using similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004A version). Besides, a high-resolution mass spectrometer was used to identify flavonoids in S. indica. A total of 27 flavonoids in S. indica were identified. And a series of fragmentation regularities were obtained, which could be used for the identification of other flavonoids. Therefore, the established liquid chromatography-tandem mass spectrometer method could be successfully utilized for the quality control of S. indica. @@@ [GRAPHICS] @@@ .

• 出版日期2017
• 单位航天中心医院; 承德医学院