A sensitive and selective chemosensor for GSSG detection based on the recovered fluorescence of NDPA-Fe3O4@SiO2-Cu(II) nanomaterial

作者：Ma Ya; Zheng Baozhan; Zhao Yan; Yuan Hongyan; Cai Yuqing; Du CJuan^*; Xiao Dan

来源：Biosensors and Bioelectronics, 2013, 48: 138-144.

DOI：10.1016/j.bios.2013.04.006

• Chemosensor
• Nanomaterial
• Fluorescence
• GSSG

A sensitive and selective sensor for oxidized glutathione (GSSG) detection based on the recovered fluorescence of naphthalimide-DPA (NDPA)-Fe3O4@SiO2-Cu(II) system is reported. NDPA-Fe3O4@SiO2 was characterized by X-ray power diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectra (FT-IR) and fluorophotometry. The fluorescence of NDPA-Fe3O4@SiO2 could be quenched by Cu2+ due to the coordination of Cu2+ with the tridentate receptor DPA. This coordination process reduced the electron-donating ability of the nitrogen atom in the DPA moiety, thus suppressing the internal charge transfer (ICE) process in NDPA-Fe3O4@SiO2. In the presence of GSSG, the fluorescence of NDPA-Fe3O4@SiO2-Cu(II) was recovered because of strong coordination of Cu2+ with GSSG, which promoted the decomplexation between NDPA-Fe3O4@SiO2 and Cu2+, and enhanced the ICE process. The NDPA-Fe3O4@SiO2-Cu(II) nanomaterial exhibited high sensitivity towards GSSG, and a good linear relationship was obtained from 5 nM to 60 mu M. The limit of detection, based on a signal-to-noise ratio of 3, was 50 pM. In addition, the presence of magnetic Fe3O4 nanoparticles (NPs) in NDPA-Fe3O4@SiO2 NPs would also facilitate the magnetic separation of NDPA-Fe3O4@SiO2 from the solution. Through the use of added internal standards, we successfully determined the concentration of GSSG in HEK 293 cell lysate to be 1.15 mu M by the prepared chemsensor NDPA-Fe3O4@SiO2-Cu(II). The proposed method is anticipated to fabricate other sensitive fluorescence sensors based on organic inorganic hybrid magnetic nanoparticles.

• 出版日期2013-10-15
• 单位四川大学