A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons

作者：Leonard R; Kolarich D; Paschinger K; Altmann F; Wilson IBH^*

来源：Plant Molecular Biology, 2004, 55(5): 631-644.

DOI：10.1007/s11103-004-1558-3

• fucosyltransferase
• glycan
• glucosyltransferase
• monocotyledon
• N-acetylglucosaminyltransferase
• oligosaccharide
• rice
• xylosyltransferase

The recent draft sequencing of the rice (Oryza sativa) genome has enabled a genetic analysis of the glycosylation capabilities of an agroeconomically important group of plants, the monocotyledons. In this study, we have not only identified genes putatively encoding enzymes involved in N-glycosylation, but have examined by MALDI-TOF MS the structures of the N-glycans of rice and other monocotyledons (maize, wheat and dates; Zea mays, Triticum aestivum and Phoenix dactylifera); these data show that within the plant kingdom the types of N-glycans found are very similar between monocotyledons, dicotyledons and gymnosperms. Subsequently, we constructed expression vectors for the key enzymes forming plant-typical structures in rice, N-acetylglucosaminyltransferase I (GlcNAc-TI; EC 2.4.1.101), core alpha1,3-fucosyltransferase (FucTA; EC 2.4.1.214) and beta1,2-xylosyltransferase (EC 2.4.2.38) and successfully expressed them in Pichia pastoris. Rice GlcNAc-TI, FucTA and xylosyltransferase are therefore the first monocotyledon glycosyltransferases involved in N-glycan biosynthesis to be characterised in a recombinant form.

• 出版日期2004-7