Advancing formaldehyde cross-linking towards quantitative proteomic applications

作者：Klockenbusch Cordula; O'Hara Jane E; Kast Juergen^*

来源：Analytical and Bioanalytical Chemistry, 2012, 404(4): 1057-1067.

DOI：10.1007/s00216-012-6065-9

• FFPE
• Formaldehyde cross-linking
• Protein-protein interactions
• Mass spectrometry

Formaldehyde is a key fixation reagent. This review explores its application in combination with qualitative and quantitative mass spectrometry (MS). Formalin-fixed and paraffin-embedded (FFPE) tissues form a large reservoir of biologically valuable samples and their investigation by MS has only recently started. Furthermore, formaldehyde can be used to stabilise protein-protein interactions in living cells. Because formaldehyde is able to modify proteins, performing MS analysis on these samples can pose a challenge. Here we discuss the chemistry of formaldehyde cross-linking, describe the problems of and progress in these two applications and their common aspects, and evaluate the potential of these methods for the future.

• 出版日期2012-9