Degalactosylated/Desialylated Bovine Colostrum Induces Macrophage Phagocytic Activity Independently of Inflammatory Cytokine Production

作者：Uto Yoshihiro^*; Kawai Tomohito; Sasaki Toshihide; Hamada Ken; Yamada Hisatsugu; Kuchiike Daisuke; Kubo Kentaro; Inui Toshio; Mette Martin; Tokunaga Ken; Hayakawa Akio; Go Akiteru; Oosaki Tomohiro

来源：Anticancer Research, 2015, 35(8): 4487-4492.

• degalactosylated/desialylated bovine colostrum
• GcMAF
• macrophage phagocytic activity
• mouse peritoneal macrophage
• mouse intestinal macrophage

Background/Aim: Colostrum contains antibodies, such as immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM), and, therefore, has potent immunomodulating activity. In particular, IgA has an O-linked sugar chain similar to that in the group-specific component (Gc) protein, a precursor of the Gc protein-derived macrophage-activating factor (GcMAF). In the present study, we investigated the macrophage-activating effects of degalactosylated/desialylated bovine colostrum. Results: We detected the positive band in degalactosylated/desialylated bovine colostrum by western blotting using Helix pomatia agglutinin lectin. We also found that degalactosylated/desialylated bovine colostrum could significantly enhance the phagocytic activity of mouse peritoneal macrophages in vitro and of intestinal macrophages in vivo. Besides, degalactosylated/desialylated bovine colostrum did not mediate the production of inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta). Conclusion: Similar to the use of GcMAF, degalactosylated/desialylated bovine colostrum can be used as a potential macrophage activator for various immunotherapies.

• 出版日期2015-8