A new method for concentration analysis of bacterial endotoxins in perfluorocarbon

作者：Chen Dan Dan; Feng Xiao Ming; Wang Chun Ren^*; Huang Qing Quan; Yang Zhao Peng; Meng Qing Yuan

来源：Frontiers of Materials Science, 2014, 8(4): 399-402.

DOI：10.1007/s11706-014-0267-3

• perfluorocarbon
• bacterial endotoxin
• endotoxin recovery

This communication demonstrates the feasibility of the gel-clot method for the analysis of bacterial endotoxins in water extracts of perfluorocarbon which is a water insoluble liquid medical device. Perfluorocarbon (10 mL) was shaken with 10mL water for 15 min at 2000 r/min and the endotoxin present was extracted to the aqueous phase without interference inhibition/enhancement of the product and the recovery of endotoxin added to perfluorocarbon was determined. A validation study confirmed that endotoxins presented in perfluorocarbon pass over into the aqueous phase at concentrations of 20, 10 and 5 EU/mL with recoveries from 86.8% to 96.8%. Therefore, the gel-clot test is suitable for detecting bacterial endotoxins in perfluorocarbon which is a water insoluble medical device.

• 出版日期2014-12
• 单位清华大学