Differential microRNA expression profiling and target gene prediction in the muscle tissues of clenbuterol-fed Chinese miniature swine

作者：Tian, M.; He, X.; Wang, W.; Liu, D.^*; Meng, Q.^*

来源：Acta Agriculturae Scandinavica Section A-Animal Science, 2017, 67(1-2): 9-14.

DOI：10.1080/09064702.2017.1332094

• Chinese miniature swine
• clenbuterol
• microRNA
• muscle

MicroRNAs (miRNAs) regulate gene expression by inhibiting the translation of target RNA. Clenbuterol (CLB) is a -adrenergic agonist that has the ability to modify muscle characteristics by promoting protein synthesis and inhibiting the growth of fat. To determine the expression profile of miRNAs in the muscle of CLB-treated Chinese miniature swine and to predict their target genes, 13 microRNAs were detected and analyzed. We found that miR-133, miR-206, and miR-1 exhibited the highest expression, although no difference in expression levels was observed between the control and the treatment groups, whereas miR-29a and miR-29b were differentially expressed in the CLB-treated muscles. Furthermore, we analyzed the relationship between miR-29a/b expression, muscle growth, and candidate target genes, and determined that the expression of the four target genes (HnRNPF, SWI/SNF, TPM1, and LPL) was regulated by miR-29a and miR-29b and were functionally related to muscle development.

• 出版日期2017
• 单位黑龙江省农业科学院; 中国农业大学