Antiproliferative Effects of Adiponectin on Human Trophoblastic Cell Lines JEG-3 and BeWo

作者：Benaitreau Delphine; Dieudonne Marie Noelle^*; Dos Santos Esther; Leneveu Marie Christine; de Mazancourt Philippe; Pecquery Rene

来源：Biology of Reproduction, 2009, 80(6): 1107-1114.

DOI：10.1095/biolreprod.108.070573

• adiponectin
• adiponectin receptors
• BeWo
• cytokines
• implantation
• JEG-3
• placenta
• proliferation
• signal transduction
• trophoblast

During embryo implantation, a complex dialog exists between the mother and the fetus. However, little is known about the molecules that participate in this process. Among various factors secreted at the maternal-fetal interface, the adipose tissue-derived leptin is now considered a placental growth factor. Adiponectin is another adipocyte-derived signaling molecule known to exert antiproliferative effects in various cell types. In this work, we studied adiponectin sensitivity and effects on JEG-3 and BeWo choriocarcinoma cell lines. First, we showed that JEG-3 and BeWo cells express the specific adiponectin receptors ADIPOR1 and ADIPOR2 and respond to human recombinant adiponectin by AMP-activated protein kinase (PRKA, also known as AMPK) activation. Second, we demonstrated that adiponectin induces a reduction in cell number and in [(3)H]-thymidine incorporation, demonstrating that adiponectin has antiproliferative effects on trophoblastic cells. Furthermore, these effects of adiponectin seem to be, at least in part, mediated by the mitogen-activated protein kinase (MAPK) and phosphoinositide-3-kinase (PI3K) signaling pathways. We describe herein the direct effects of adiponectin in the control of trophoblastic cell proliferation.

• 出版日期2009-6