科研之友
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摘要
Purpose: To investigate the accuracy of I-125 radiolabeling to quantitatively determine the deposition of protein onto various commercially available contact lens (CL) materials. Methods: Commercially available silicone hydrogel and conventional hydrogel CL materials were examined for times ranging from 10s to 1 week. Adsorption of free I-125 was measured directly for the CL. The use of dialyzing labeled proteins and/or using NaI to compete with free I-125 uptake was investigated as ways to minimize effects due to free I-125. Results: At all time points and with all lens materials, there was 0.3 mu g/lens or greater of apparent mass attributable to free I-125 uptake. Dialyzing labeled proteins significantly reduced free I-125 uptake for all materials investigated. The benefit of using dialyzed protein was most prominent at shorter times, as free I-125 is continuously generated over time. Using NaI can reduce free I-125 uptake for some lens materials, but this is shown to directly affect protein deposition on some materials. Conclusions: Periodic replenishment of incubation solutions with freshly dialyzed labeled protein to limit free I-125 generation is recommended, but the incorporation of NaI onto the buffer solution is not. Irrespective of the exact procedure to limit free I-125 uptake, extra steps must be performed to quantify the amount of I-125 adsorbed onto contact lens materials, to determine thresholds of confidence with respect to the actual protein deposition that occurs.
- 出版日期2016
