Differential Chlorate Inhibition of Chaetomium globosum Germination, Hyphal Growth, and Perithecia Synthesis

作者：Biles Charles L^*; Wright Desiree; Fuego Marianni; Guinn Angela; Cluck Terry; Young Jennifer; Martin Markie; Biles Josiah; Poudyal Shubhra

来源：Mycopathologia, 2012, 174(5-6): 475-487.

DOI：10.1007/s11046-012-9572-5

• Chaetomium sp.
• Fungal inhibition
• Mold
• Potassium chlorate
• Chlorate toxicity
• Spore germination

Chaetomium globosum Kunze:Fr is a dermatophytic, dematiaceous fungus that is ubiquitous in soils, grows readily on cellulolytic materials, and is commonly found on water-damaged building materials. Chlorate affects nitrogen metabolism in fungi and is used to study compatibility among anamorphic fungi by inducing nit mutants. The effect of chlorate toxicity on C. globosum was investigated by amending a modified malt extract agar (MEA), oat agar, and carboxymethyl cellulose agar (CMC) with various levels of potassium chlorate (KClO3). C. globosum perithecia production was almost completely inhibited (90-100 %) at low levels of KClO3 (0.1 mM) in amended MEA. Inhibition of perithecia production was also observed on oat agar and CMC at 1 and 10 mM, respectively. However, hyphal growth in MEA was only inhibited 20 % by 0.1-100 mM KClO3 concentrations. Hyphal growth was never completely inhibited at the highest levels tested (200 mM). Higher levels of KClO3 were needed on gypsum board to inhibit perithecia synthesis. In additional experiments, KClO3 did not inhibit C. globosum, Fusarium oxysporum, Aspergillus niger, Penicillum expansum, and airborne fungal spore germination. The various fungal spores were not inhibited by KClO3 at 1-100 mM levels. These results suggest that C. globosum perithecia synthesis is more sensitive to chlorate toxicity than are hyphal growth and spore germination. This research provides basic information that furthers our understanding about perithecia formation and may help in developing control methods for fungal growth on building materials.

• 出版日期2012-12