Phenolic compounds from Viscum album tinctures enhanced antitumor activity in melanoma murine cancer cells

作者：de Oliveira Melo Michelle Nonato; Oliveira Adriana Passos^*; Wiecikowski Adalgisa Felippe; Carvalho Renato Sampaio; Castro Juliana de Lima; Gomes de Oliveira Felipe Alves; Gualberto Pereira Henrique Marcelo; da Veiga Venicio Feo; Alves Capella Marcia Marques; Rocha Leandro; Holandino Carla

来源：Saudi Pharmaceutical Journal, 2018, 26(3): 311-322.

DOI：10.1016/j.jsps.2018.01.011

• Viscum album
• Mistletoe
• Lignans
• Phenolic compounds
• Antitumoral

Cancer is one of the biggest problems in public health worldwide. Plants have been shown important role in anticancer research. Viscum album L. (Santalaceae), commonly known as mistletoe, is a semi-parasitic plant that grows on different host trees. In complementary medicine, extracts from European mistletoe (Viscum album L.) have been used in the treatment of cancer. The study was conducted to identify chemical composition and antitumor potential of Viscum album tinctures. Chemical analysis performed by high resolution chromatography equipped with high resolution mass spectrometer identified caffeic acid, chlorogenic acid, sakuranetin, isosakuranetin, syringenin 4-O-glucoside, syringenin 4-O-apiosyl-glucoside, alangilignoside C and ligalbumoside A compounds. Some of these compounds are probably responsible for the reduction of tumoral cellular growth in a dose-dependent manner. It was observed that melanoma murine cells (B16F10) were more sensitive to V. album tinctures than human leukaemic cells (K562), besides non-tumoral cells (MA-104) had a much lower cytotoxicity to them. Apoptotic-like cells were observed under light microscopy and were confirmed by a typical DNA fragmentation pattern. Additionally, flow cytometry results using Annexin-V/FITC permitted to quantify increased expression of early and late apoptotic markers on tumoral cells, confirming augmented Sub G0 population, which was probably associated with a consistent decrease in G1, and an increase in S or G2/M populations. Results indicate the chemical composition of V. album tinctures influences the mechanisms of in vitro tumoral cell death, suggesting a potential use in cancer pharmacotherapy research.

• 出版日期2018-3