A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues

作者:Rodriguez Rigueiro Teresa; Valladares Ayerbes Manuel; Haz Conde Mar; Blanco Moises; Aparicio Guadalupe; Fernandez Puente Patricia; Blanco Francisco J; Jose Lorenzo Ma; Aparicio Luis A; Figueroa Angelica*
来源:Proteomics, 2011, 11(12): 2555-2559.
DOI:10.1002/pmic.201000809

摘要

Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.

  • 出版日期2011-6