Pinoresinol Diglucoside is Screened as a Putative alpha-Glucosidase Inhibiting Compound in Actinidia arguta leaves

作者：Kwon Dodan; Kim Gyoung Deuck; Kang Wonseok; Park Jeong Eun; Kim Song Hee; Choe Eunok; Kim Jung In; Auh Joong Hyuck^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2014, 57(4): 473-479.

DOI：10.1007/s13765-014-4167-0

• Actinidia arguta
• alpha-glucosidase
• liquid chromatography mass spectrometry
• pinoresinol diglucoside

Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the alpha-glucosidase inhibitory compounds in A. arguta were screened and identified through alpha-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting alpha-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as alpha-glucosidase inhibitory agents, pinoresinol diglucoside was proposed to be a putative key compound for alpha-glucosidase inhibition in A. arguta. This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural alpha-glucosidase inhibitor.

• 出版日期2014-8