alpha(V)beta(3) Integrin regulates astrocyte reactivity

作者：Lagos Cabre Raul; Alvarez Alvaro; Kong Milene; Burgos Bravo Francesca; Cardenas Areli; Rojas Mancilla Edgardo; Perez Nunez Ramon; Herrera Molina Rodrigo; Rojas Fabiola; Schneider Pascal; Herrera Marschitz Mario; Quest Andrew F G; van Zundert Brigitte; Leyton Lisette^*

来源：Journal of Neuroinflammation, 2017, 14(1): 194.

DOI：10.1186/s12974-017-0968-5

• Integrins
• Inflammation
• Reactive astrocytes
• Amyotrophic lateral sclerosis
• Cell migration

Background: Neuroinflammation involves cytokine release, astrocyte reactivity and migration. Neuronal Thy-1 promotes DITNC1 astrocyte migration by engaging alpha(V)beta(3) Integrin and Syndecan-4. Primary astrocytes express low levels of these receptors and are unresponsive to Thy-1; thus, inflammation and astrocyte reactivity might be necessary for Thy-1-induced responses.
Methods: Wild-type rat astrocytes (TNF-activated) or from human SOD1(G93A) transgenic mice (a neurodegenerative disease model) were used to evaluate cell migration, Thy-1 receptor levels, signaling molecules, and reactivity markers.
Results: Thy-1 induced astrocyte migration only after TNF priming. Increased expression of alpha(V)beta(3) Integrin, Syndecan-4, P2X7R, Pannexin-1, Connexin-43, GFAP, and iNOS were observed in TNF-treated astrocytes. Silencing of beta(3) Integrin prior to TNF treatment prevented Thy-1-induced migration, while beta(3) Integrin over-expression was sufficient to induce astrocyte reactivity and allow Thy-1-induced migration. Finally, hSOD1(G93A) astrocytes behave as TNF-treated astrocytes since they were reactive and responsive to Thy-1.
Conclusions: Therefore, inflammation induces expression of alpha(V)beta(3) Integrin and other proteins, astrocyte reactivity, and Thy-1 responsiveness. Importantly, ectopic control of beta(3) Integrin levels modulates these responses regardless of inflammation.

• 出版日期2017-9-29