Activating Water: Efficient Intramolecular General Base Catalysis of the Hydrolysis of a Phosphate Triester

作者：Kirby Anthony J; Medeiros Michelle; Oliveira Pedro S M; Brandao Tiago A S; Nome Faruk^*

来源：Chemistry - A European Journal, 2009, 15(34): 8475-8479.

DOI：10.1002/chem.200901096

• bioorganic chemistry
• enzyme catalysis
• hydrolysis
• intramolecular catalysis
• phosphate transfer

We have identified the first highly efficient intramolecular general base catalysis (IGBC) of a hydrolysis reaction, in a system where two general bases are available to assist the attack of the same nucleophilic water molecule. The suggested mechanism, available uniquely to a phosphate triester model, is readily available in enzyme active sites, and the results suggest a possible solution to the long-unsolved question of how enzymes are able to activate a water molecule to be a highly effective nucleophile

• 出版日期2009