Alpha-Tocopherol at Nanomolar Concentrations Increases the Viability of PC12 Cells under Oxidative Stress Conditions. The Effects of Modulation of Signaling Systems

作者：Sokolova T V; Rychkova M P; Zakharova I O; Voynova I V; Avrova N F^*

来源：Neurochemical Journal, 2011, 5(3): 183-190.

DOI：10.1134/S181971241103007X

• alpha-tocopherol
• nanomolar concentrations
• protective effect
• PC12 cells
• oxidative stress
• signal systems

We found that long-term preincubation of neuronal-like PC12 cells with alpha-tocopherol at micro- as well as nanomolar concentrations significantly increased cell viability under oxidative stress conditions. We discovered that the protective effect of alpha-tocopherol increases with an increase in its concentration in the 1100 nM range, while its effects at concentrations of 100 nM, 1, 10, and 100 mu M were similar when alpha-tocopherol was applied to cells 12-18 h prior to H(2)O(2). An important role in the protective effect of long-term preincubation of cells with alpha-tocopherol at various concentrations is probably related to its modulatory influence on the activities of protein kinase C, extracellular signal-activated protein kinase, and phosphatidylinositol 3-kinase. Short-term preincubation of PC12 cells with this antioxidant at nanomolar concentrations for 0.5 or 1.5 h practically did not influence cell viability, while the protective effect of alpha-tocopherol at micro- molar concentrations was probably related to its ability to terminate free-radical reactions due to direct interactions with free radicals.

• 出版日期2011-9