An improved and miniaturized analytical strategy based on mu-QuEChERS for isolation of polyphenols. A powerful approach for quality control of baby foods

作者：Casado Natalia; Perestrelo Rosa; Silva Catarina L; Sierra Isabel; Camara Jose S^*

来源：Microchemical Journal, 2018, 139: 110-118.

DOI：10.1016/j.microc.2018.02.026

• mu-QuEChERS
• Polyphenols
• Baby foods
• UHPLC-PDA
• Antioxidant capacity
• Food analysis

An improved and miniaturized quick, easy, cheap, effective, rugged and safe (mu-QuEChERS) strategy combined with ultra-high pressure liquid chromatography coupled to a photodiode array detection system (UHPLC-PDA) was developed and optimized for the determination of twelve polyphenols in different baby foods samples. The proposed analytical approach proved to be highly cost-effective and environmentally friendly, since it showed good extraction efficiency using few amounts of sample (0.3 g), organic solvents (1000 mu L ), clean-up sorbents (87.5 mg) and partitioning salts (0.2 g), producing minimal waste disposal and reducing analysis time. Method performance was validated in terms of selectivity, linearity, limits of detection and quantification, matrix effects, precision and accuracy using different baby food matrices according to their composition. The overall average recoveries ranged from 71 to 100% with relative standard deviations lower than 6% (n = 18). The method was successfully applied to commercial baby food samples (including pureed solids, juices and porridges), and their total phenolic content (TPC) and antioxidant capacity (RSA) were also investigated. Results allowed characterizing the abundance of the selected polyphenols in the samples, and the highest RSA and TPC were found in the fruit-based baby foods. Thus, this work represents a first approach to determine the nutritional quality of these products.

• 出版日期2018-6