A B-Myb complex containing clathrin and filamin is required for mitotic spindle function

作者：Yamauchi Tomohiro; Ishidao Takefumi; Nomura Teruaki; Shinagawa Toshie; Tanaka Yasunori; Yonemura Shigenobu; Ishii Shunsuke^*

来源：The EMBO Journal, 2008, 27(13): 1852-1862.

DOI：10.1038/emboj.2008.118

• B-Myb
• clathrin
• filamin
• genome instability
• mitotic spindle

B-Myb is one member of the vertebrate Myb family of transcription factors and is ubiquitously expressed. B-Myb activates transcription of a group of genes required for the G2/M cell cycle transition by forming the dREAM/Myb MuvB-like complex, which was originally identified in Drosophila. Mutants of zebrafish B-myb and Drosophila myb exhibit defects in cell cycle progression and genome instability. Although the genome instability caused by a loss of B-Myb has been speculated to be due to abnormal cell cycle progression, the precise mechanism remains unknown. Here, we have purified a B-Myb complex containing clathrin and filamin (Myb-Clafi complex). This complex is required for normal localization of clathrin at the mitotic spindle, which was previously reported to stabilize kinetochore fibres. The Myb-Clafi complex is not tightly associated with the mitotic spindles, suggesting that this complex ferries clathrin to the mitotic spindles. Thus, identification of the Myb-Clafi complex reveals a previously unrecognized function of B-Myb that may contribute to its role in chromosome stability, possibly, tumour suppression.

• 出版日期2008-7-9